RuP2 Pool Size Indicated by CO2 Assimilation Following the Abrupt Loss of Light

Cary, J.W. and Kleinkopf, G.E. (1986) RuP2 Pool Size Indicated by CO2 Assimilation Following the Abrupt Loss of Light. Photosynthesis Research. 8:123-132.

PDF 574.pdf Download (269kB)

Abstract

Measurement of the changes in CO2 uptake by single leaves following the
abrupt onset of darkness were made on sugarbeets (Beta vulgaris L.) and (Phaseolus
vulgaris L.) The shape of the CO2 dark response curve was analyzed with respect to the
reaction kinetics of CO2 RuP2 and RuP2 carboxylase. It was concluded that the net
uptake of CO2 in the dark from a 1% O2 atmosphere can be approximately related to
the pool size of the RuP2 substrate in the chloroplasts of C3 plants. This information
was combined with CO2 levels and decay rates of the response curves to infer changes
in carboxylase activity. Preliminary data are presented showing the relative concentration
changes in RuP2 as light intensity decreases and as water stress increases. The
method may prove useful in studies of plant response to environmental stresses.

Item Type:	Article
NWISRL Publication Number:	0574
Subjects:	Mass Import - unclassified
Depositing User:	Dan Stieneke
Date Deposited:	20 Nov 2010 21:52
Last Modified:	13 Jan 2017 20:30
Item ID:	456
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/456