Root microbiome and metabolome traits associated with improved post-harvest root storage for sugar beet breeding lines under southern Idaho conditions

Majumdar, R. and Strausbaugh, C.A. and Kandel, Shyam L. (2024) Root microbiome and metabolome traits associated with improved post-harvest root storage for sugar beet breeding lines under southern Idaho conditions. International Journal of Molecular Sciences. 25(23):1-25. 26 November 2024.

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Abstract

Post-harvest storage loss in sugar beets due to root rot and respiration can cause >20% sugar loss. Breeding strategies focused on factors contributing to improved post-harvest storage quality are of great importance to prevent losses. Using 16S rRNA and ITS sequencing and sugar beet mutational breeding lines with high disease resistance (R), along with a susceptible (S) commercial cultivar, the role of root microbiome and metabolome in storage performance was investigated. The R lines in general showed higher abundance of bacterial phyla, Patescibacteria at M time point, and Cyanobacteria, and Desulfobacterota at L time point. Amongst fungal phyla, Basidiomycota (including Athelia) and Ascomycota were predominant in diseased samples. Linear discriminant analysis Effect Size (LEfSe) identified bacterial taxa such as Micrococcales, Micrococcaceae, Bacilli, Glutamicibacter, Nesterenkonia, and Paenarthrobacter as putative biomarkers associated with resistance in the R lines. Further functional enrichment analysis showed higher abundance of bacteria such as those related to the super pathway of pyrimidine deoxyribonucleosides degradation, L-tryptophan biosynthesis at M and L, and fungi such as those associated with the biosynthesis of L-iditol 2-dehydrogenase at L in the R lines. Metabolome analysis of the roots revealed higher enrichment of pathways associated with arginine, proline, alanine, aspartate, and glutamate metabolism at M, and in addition beta-alanine, butanoate metabolism at L in the R lines. Correlation analysis between microbiome and metabolites indicated that root biochemical composition such as nitrogen containing secondary metabolites may regulate relative abundances of key microbial candidates contributing to better post-harvest storage.

Item Type:	Article
NWISRL Publication Number:	1800
Subjects:	Irrigated crops > Sugarbeet > Resistance Irrigated crops > Sugarbeet > Storage Irrigated crops > Sugarbeet
Depositing User:	Users 11 not found.
Date Deposited:	26 Nov 2024 17:24
Last Modified:	26 Nov 2024 17:24
Item ID:	1842
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/1842