Partition Column Chromatography for Quantitating Effects of Fertilization on Plant Acids

Prior, R.L. and Grunes, D.L. and Patterson, R.P. and Smith, F.W. and Mayland, H.F. and Visek, W.J. (1972) Partition Column Chromatography for Quantitating Effects of Fertilization on Plant Acids. Journal of Agricultural and Food Chemistry. 21:73-77.

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Abstract

A method for determining organic acid levels in
biological materials by silica gel partition column
chromatography (pcc) is described. It uses tetrabromophenolphthalein
ethyl ester, which has a
high molar absorbency, for quantitating the acids
in the column effluent. The concentrations of
several organic acids were determined in two
species of crested wheatgrass, Nordan (Agropyron
desertorum) and Fairway (A. cristatum), grown.
under different conditions of K fertilization. For
comparison, aconitic, citric, and malic acids were
also determined by polarography, spectrophotometry,
and fluorometry, respectively. Results obtained
by these methods agreed with the data obtained
by pcc. The concentration of trans-aconitic
acid averaged about 96 and 21 mequiv/kg of
dry matter in Nordan and Fairway, respectively,
when grown in the greenhouse without K. K fertilization
(312 kg/ha) approximately doubled the
trans-aconitic acid concentration in both species.
Fairway contained 363 mequiv/kg of malic acid,
while Nordan contained 280 mequiv/kg. K fertilization
increased these concentrations to 611 and
446 mequiv/kg, respectively. Citric acid was increased
by K fertilization, but there was no significant
difference between species and in no case
was the citric acid concentration greater than 80
mequiv/kg of dry matter.

Item Type:	Article
NWISRL Publication Number:	0245
Subjects:	Research methodology Mass Import - autoclassified (may be erroneous)
Depositing User:	Dan Stieneke
Date Deposited:	20 Nov 2010 21:51
Last Modified:	16 Feb 2017 23:00
Item ID:	232
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/232