Pathogenicity and real-time PCR detection of Fusarium spp. in wheat and barley roots

Strausbaugh, C.A. and Overturf, K. and Koehn, A.C. (2005) Pathogenicity and real-time PCR detection of Fusarium spp. in wheat and barley roots. Canadian Journal of Plant Pathology. 27:430-438.

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Abstract

The pathogenicity of five Fusarium spp. frequently isolated from wheat and barley roots in southern Idaho
was investigated during four growth-chamber experiments and two field studies. A real-time PCR assay for quantifying
the presence of E culmorum from infected root tissue was also developed based on nucleotide sequence for the tri5
gene. Fusarium culmorum, followed by E acuminatum and E reticulatum, resulted in the largest root lesions and
percent infected root area. However, E semitectum, followed by E acuminatum and E equiseti, had the greatest impact
on total root length. The TaqMan-based real-time assay was able to quantify E culmorum in root tissue from both
growth-chamber and field studies down to 61 pg. The assay also detected E pseudograminearum and E graminearum
but could not distinguish among these three Fusarium spp

Item Type:	Article
NWISRL Publication Number:	1167
Subjects:	Irrigated crops > Small grain > Root rot
Depositing User:	Users 3 not found.
Date Deposited:	24 Mar 2008 21:24
Last Modified:	01 Nov 2016 16:06
Item ID:	19
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/19