Combined Omics Approaches Reveal Distinct Mechanisms of Resistance and/or Susceptibility in Sugar Beet Double Haploid Genotypes at Early Stages of Beet Curly Top Virus Infection

Galewski, Paul J. and Majumdar, R. and Strausbaugh, C.A. and Eujayl, Imad A. (2023) Combined Omics Approaches Reveal Distinct Mechanisms of Resistance and/or Susceptibility in Sugar Beet Double Haploid Genotypes at Early Stages of Beet Curly Top Virus Infection. International Journal of Molecular Sciences. 24(15013):1-26. 9 October 2023.

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Abstract

Sugar beet is highly susceptible to Beet curly top virus (BCTV) which significantly reduces yield and sugar production in the semi-arid growing regions worldwide. Sources of genetic resistance to BCTV is highly limited and primarily dependent upon seed treatment with neonicotinoids, the use of which is gradually being restricted. Through double haploid production and genetic selection, we have developed BCTV resistant breeding lines. Using BCTV resistant (R) [KDH13; Line 13, and KDH4-9; Line 4] and susceptible (S) [KDH19-17; Line 19] lines, beet leafhopper meditated natural infection, mRNA/sRNA sequencing, and metabolite analyses we demonstrate potential mechanisms of resistance against the virus. At early infection stages (2- and 6-days post inoculation), examples of differentially expressed genes highly up-regulated in the ‘R’ lines (vs. ‘S’) include EL10Ac5g10437 (inhibitor of trypsin and hageman factor), EL10Ac6g14635 (jasmonate induced protein), EL10Ac3g06016 (ribosome related), EL10Ac2g03119 (unknown) etc. Pathway enrichment analysis showed differentially expressed genes predominantly involved with peroxisome, amino acids metabolism, fatty acid degradation, amino/nucleotide sugar metabolism etc. Metabolite analysis revealed significantly higher amounts of isoflavonoid O-glycosides, flavonoid 8-C glycosides, triterpenoid, iridoid-O-glycosides in the leaves of the ‘R’ lines (vs. ‘S’). The data presented here suggest a combination of transcriptional regulation and production of antiviral metabolites might contribute to BCTV resistance. In addition, genome divergence among BCTV strains differentially affects the production of small non-coding RNAs (sncRNAs) and small peptides which may potentially affect pathogenicity and disease symptom development.

Item Type:	Article
NWISRL Publication Number:	1768
Subjects:	Irrigated crops > Sugarbeet > Curly top Irrigated crops > Sugarbeet > Genetics Irrigated crops > Sugarbeet > Resistance Irrigated crops > Sugarbeet
Depositing User:	Users 11 not found.
Date Deposited:	10 Oct 2023 15:45
Last Modified:	10 Oct 2023 15:45
Item ID:	1809
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/1809