Cell wall degrading enzymes originating from rhizoctonia solani increase sugar beet root damage in the presence of leuconostoc mesenteroides

Majumdar, R. and Strausbaugh, C.A. and Galewski, Paul J. and Minocha, Rakesh and Rogers, C.W. (2022) Cell wall degrading enzymes originating from rhizoctonia solani increase sugar beet root damage in the presence of leuconostoc mesenteroides. International Journal of Molecular Sciences. 23(3):1-25. 25 January 2022. Available: https://doi.org/10.3390/ijms23031366

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Abstract

Sugar beet crown and root rot caused by Rhizoctonia solani is a major yield constraint. Root rot is highly increased when R. solani and Leuconostoc mesenteroides co-infect roots. We hypothesized that the absence of plant cell wall degrading enzymes in L. mesenteroides and their supply by R. solani during close contact, causes increased damage. In planta root inoculation with or without cell wall degrading enzymes showed greater rot when L. mesenteroides was combined with cellulase (49 mm rot), polygalacturonase (48 mm), and pectin lyase (35 mm) versus these enzymes (0–11 mm), R. solani (13 mm), and L. mesenteroides (22 mm) individually. Carbohydrate analysis revealed increase in simpler carbohydrates namely glucose + galactose, and fructose in the infected roots versus mock control, possibly due to the degradation of complex cell wall carbohydrates. Expression of R. solani cellulase, polygalacturonase, and pectin lyase genes during root infection corroborated well with the enzyme data. Global mRNAseq analysis identified candidate genes and highly co-expressed gene clusters (in all 3 organisms) that might be critical in host plant defense and pathogenesis. Future targeting of R. solani cell wall degrading enzymes could be an effective strategy to mitigate root damage during interaction with L. mesenteroides.

Item Type:	Article
NWISRL Publication Number:	1720
Subjects:	Irrigated crops > Sugarbeet > Genetics Irrigated crops > Sugarbeet > Root rots Irrigated crops > Sugarbeet
Depositing User:	Users 6 not found.
Date Deposited:	27 Jan 2022 19:49
Last Modified:	27 Jan 2022 19:49
Item ID:	1758
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/1758