Regulatory roles of small non-coding RNAs in sugar beet resistance against beet curly top virus

Majumdar, R. and Galewski, Paul J. and Eujayl, Imad A. and Minocha, Rakesh and Vincill, E.D. and Strausbaugh, C.A. (2022) Regulatory roles of small non-coding RNAs in sugar beet resistance against beet curly top virus. Frontiers in Plant Science. 12:1-22. 10 January 2022. Available: https://doi.org/10.3389/fpls.2021.780877

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Abstract

Beet curly top virus (BCTV) mediated yield loss in sugar beets is a major problem worldwide. The circular single-stranded DNA virus is transmitted by the beet leafhopper. Genetic sources of BCTV resistance in sugar beet are limited and commercial cultivars rely on chemical treatments versus durable genetic resistance. Phenotypic selection and double haploid production have resulted in sugar beet germplasm (KDH13-13 and KDH4-9-4) that are highly resistant to BCTV. The molecular mechanism of resistance to the virus is unknown, especially the role of small noncoding RNAs (sncRNAs) during early plant-viral interaction. Using the resistant lines along with a susceptible line (KDH19-17; 19), we demonstrate the role of sugar beet miRNAs in BCTV resistance during early infection stages when symptoms are not yet visible. The differentially expressed miRNAs altered the expression of their corresponding target genes such as pyruvate dehydrogenase (EL10Ac1g02046), carboxylesterase (EL10Ac1g01087), serine/threonine protein phosphatase (EL10Ac1g01374), and LRR receptor-like (EL10Ac7g17778), that were highly expressed in the resistant lines versus susceptible lines. Pathway enrichment analysis of the miRNA target genes showed an enrichment of genes involved in glycolysis/gluconeogenesis, galactose metabolism, starch, and sucrose metabolism to name a few. Carbohydrate analysis revealed altered glucose, galactose, fructose, and sucrose concentration in the infected leaves of resistant versus susceptible lines. We also demonstrate differential regulation of BCTV derived sncRNAs in the resistant versus susceptible lines that target sugar beet genes such as LRR (EL10Ac1g01206), 7-deoxyloganetic acid glucosyltransferase (EL10Ac5g12605), and transmembrane emp24 domain containing (EL10Ac6g14074) and altered their expression. In response to viral infection, we found that plant derived miRNAs targeted BCTV capsid protein/replication related genes and showed differences in expression among resistant and susceptible lines. The data presented here demonstrate the contribution of miRNA mediated regulation of metabolic pathways and cross-kingdom RNAi in sugar beet BCTV resistance.

Item Type:	Article
NWISRL Publication Number:	1719
Subjects:	Irrigated crops > Sugarbeet > Curly top Irrigated crops > Sugarbeet > Genetics Irrigated crops > Sugarbeet > Resistance Irrigated crops > Sugarbeet
Depositing User:	Users 6 not found.
Date Deposited:	10 Jan 2022 14:52
Last Modified:	10 Jan 2022 14:52
Item ID:	1757
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/1757