Beet curly top resistance in USDA-ARS Ft. Collins germplasm, 2016

Panella, Leonard W. and Strausbaugh, C.A. (2017) Beet curly top resistance in USDA-ARS Ft. Collins germplasm, 2016. Plant Disease Management Reports. 11:V084. 7 March 2017.

Text 1593.pdf Download (158kB)

Abstract

Fifty-one sugar beet (Beta vulgaris L.) germplasm lines produced by the USDA-ARS Ft. Collins sugar beet program and three commercial check cultivars [SV2012RR (susceptible) and HM PM90 and Beta G6040 (resistant)] were screened for resistance to Beet curly top virus (BCTV). The curly top evaluation was conducted at the USDA-ARS North Farm in Kimberly, ID which has Portneuf silt loam soil and had been in barley in 2015. The field was plowed in the fall and in the spring, it was fertilized (90 lb N and 110 lb P2O5/A) and roller harrowed on April 4. The germplasm was planted (density of 142,560 seeds/A) on May 16. The plots were two rows 10 ft long with 22-in row spacing and arranged in a randomized complete block design with four replications. The field was sprinkler irrigated, cultivated, and hand weeded as necessary. Plant populations were thinned to about 47,500 plants/A on June 16. Plants were inoculated at the four- to six-leaf growth stage on June 20th with approximately six viruliferous (contained at least the following BCTV strains: Cal/Logan, CO, Severe, and Worland) beet leafhoppers per plant. The beet leafhoppers were redistributed three times a day during the first two days and then twice a day for five more days by dragging a tarp through the field. The plants were sprayed on June 30th with Lorsban 4E (1.5 pints/A) to kill the beet leafhoppers. Plots were rated for foliar symptom development on July 13th using a scale of 0 to 9 (0 = healthy and 9 = dead), with the scale treated as a continuous variable (Plant Dis. 90:1539-1544). Data were analyzed in SAS using the general linear models procedure (Proc GLM), and Fisher’s protected least significant difference (LSD; a = 0.05) was used for mean comparisons. Curly top symptom development was uniform and no other disease problems were evident in the plot area. The resistant and susceptible checks performed as expected for the visual ratings. Based on the visual rating, seven entries (1, 14, 15, 32, 40, 44, and 51) were not significantly different from the resistant check. These germplasm will be retested and, if resistance is confirmed, they may be incorporated into the USDA-ARS germplasm improvement program as sources of resistance to BCTV. These results and germplasm will be accessible to interested parties through the USDA-ARS, NPGS GRIN database (http://www.ars-grin.gov/npgs/index.html).

Item Type:	Article
NWISRL Publication Number:	1593
Subjects:	Irrigated crops > Sugarbeet > Curly top Irrigated crops > Sugarbeet > Resistance Irrigated crops > Sugarbeet
Depositing User:	Users 6 not found.
Date Deposited:	22 Aug 2017 14:28
Last Modified:	22 Aug 2017 14:28
Item ID:	1645
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/1645