Pathogenicity, vegetative compatibility, and genetic diversity of verticillium dahliae isolates from sugar beet

Strausbaugh, C.A. and Eujayl, Imad A. and Martin, Frank N. (2016) Pathogenicity, vegetative compatibility, and genetic diversity of verticillium dahliae isolates from sugar beet. Canadian Journal of Plant Pathology. 38(4):492-505. 22 December 2016.

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Abstract

Verticillium wilt of sugar beet is a disease problem that has received very little attention in the literature, but has been reported to reduce sucrose production and purity. To improve our understanding of the disease, a survey of sugar beet plants with wilt symptoms in Idaho was conducted in 2007 (5 roots from each of 40 fields; 200 roots total) and 2008 (5 roots from each of 45 fields; 225 roots total). Verticillium dahliae was isolated from all plants, while Fusarium oxysporum was isolated from 19 and 21% of the plants in 2007 and 2008, respectively. From a collection of 106 V. dahliae sugar beet isolates, all isolates had the MAT1-2 mating type and 95% evaluated for vegetative compatibility group (VCG) were 4A, while 1, 1, and 3% were non-compatible, VCG 4B, and VCG 2B, respectively. All the 4A isolates had the same mitochondrial haplotype based on sequencing of the cox3 to nad6 and cox1 to rnl loci. In greenhouse pathogenicity tests on sugar beet cultivar ‘Monohikari', the VCG 4A isolates produced more foliar symptoms (P < 0.0001) than VCG 1, 1A, 2A, 2B, 3, and 4B isolates, but none of the VCGs consistently reduced root and top weight. Since V. dahliae VCG 4A strains have also been documented to be the primary cause of Verticillium wilt in potato in North America, rotating sugar beet with potato could be a concern, but unlike potatoes, sugar beet plants do not seem to die from infection.

Item Type:	Article
NWISRL Publication Number:	1589
Subjects:	Irrigated crops > Sugarbeet > Genetics Irrigated crops > Sugarbeet > Verticillium wilt Irrigated crops > Sugarbeet
Depositing User:	Users 6 not found.
Date Deposited:	22 Dec 2016 22:00
Last Modified:	26 Sep 2018 19:50
Item ID:	1632
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/1632