Beet curly top resistance of USDA-ARS National Plant Germplasm System Plant Introductions, 2010

Panella, Leonard W. and Strausbaugh, C.A. (2011) Beet curly top resistance of USDA-ARS National Plant Germplasm System Plant Introductions, 2010. Plant Disease Management Reports. 5:p. FC066.

PDF 1459.pdf Download (87kB)

Abstract

Twenty-six wild beet (Beta vulgaris subsp. maritima (L.) Arcang) accessions from the Beta collection of the USDA-ARS National Plant Germplasm System were screened for resistance to Beet severe curly top virus (BSCTV) and other closely related Curtovirus species in 2010. The curly top evaluation was conducted at the USDA-ARS North Farm in Kimberly, ID which had been in beans in 2009. The field was disked in the spring, fertilized (160 lb P2O5/A) on 7 Apr 09, sprayed with Ethotron (2 pt/A), and roller harrowed. The germplasm was planted (density of 142,560 seeds/A) on 18 May. The plots were two rows 10 ft long with 22-in row spacing and arranged in a randomized complete block design with two replications. A resistant breeding line from Betaseed, Inc., G6040, was included as a resistant check. The fields were sprinkler irrigated and hand weeded as necessary. Plant populations were thinned to about 47,500 plants/A on 19 Jun. Plants were inoculated at the four to six leaf growth stage on 23 Jun with six viruliferous beet leafhoppers per plant. The beet leafhoppers were moved twice a day (right after sunrise and just before sunset) for one week by dragging a tarp through the field. The plants were sprayed with Lorsban 4E (1.5 pints/A) on 7 Jul to kill the beet leafhoppers. The plots were rated for foliar symptom development on 15 Jul using a scale of 0-9 (0 = healthy with no sign of disease and 9 = leaves necrotic and plant dead; Mumford, D.L. 1974. Procedure for inducing curly top epidemics in field plots. J. Am. Soc. Sugar Beet Technol. 18:20-23), with disease index (DI) treated as a continuous variable. Data were analyzed using the general linear models procedure (Proc GLM-SAS), and Fisher’s protected least significant difference was used for mean comparisons.
Curly top development was uniform and other disease problems were not evident in the plot area. The PIs were a combination of annual and biennial plant types. The resistant check was not significantly more resistant than any of the 19 lowest rated lines (i.e. most resistant), which ranged from having a DI of 5.0 to having a DI of 6.0. The resistant germplasm were from a number of European countries. With the narrow range of scores (4.95 to 7.25), it will be necessary to evaluate these germplasm once more at a future date.

Item Type:	Article
NWISRL Publication Number:	1459
Subjects:	Irrigated crops > Sugarbeet > Curly top Irrigated crops > Sugarbeet
Depositing User:	Users 6 not found.
Date Deposited:	10 May 2013 18:37
Last Modified:	04 Mar 2014 15:21
Item ID:	1495
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/1495