Quantification of bacterial indicators and zoonotic pathogens in dairy wastewater ponds

Dungan, R.S. and Klein, Marcus and Leytem, A.B. (2012) Quantification of bacterial indicators and zoonotic pathogens in dairy wastewater ponds. Applied and Environmental Microbiology. 78(22):8089-8095. 22 October 2012.

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Abstract

Zoonotic pathogens in land-applied dairy wastewaters are a potential health risk. The abundance and occurrence of 10 pathogens and 3 fecal indicators was determined by quantitative real-time PCR (qPCR) in 30 dairy wastewaters from southern Idaho. Samples tested positive for Campylobacter jejuni, stx1- and eaeA-positive Escherichia coli, Listeria monocytogenes, Mycobacterium avium, and Salmonella enterica, with mean recoveries ranging from 1,000 to 10,000 genome copies per mL of wastewater. The most dominant organisms were C. jejuni and M. avium, being detected in up to 21 and 29 of 30 wastewater ponds, respectively. The qPCR detection limits for the putative pathogens in the wastewaters ranged from 16 cells mL for M. avium to 1,689 oocysts mL for Cryptosporidum. Cryptosporidium and Giardia spp., Yersinia pseudotuberculosis, and pathogenic Leptospira spp. were not detected by qPCR.

Item Type:	Article
NWISRL Publication Number:	1438
Subjects:	Manure > Chemistry Manure
Depositing User:	Users 6 not found.
Date Deposited:	26 Oct 2012 17:29
Last Modified:	26 Oct 2012 17:30
Item ID:	1473
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/1473