Evaluation of phosphorus characterization in broiler ileal digesta, manure, and litter samples: 31P-NMR vs. HPLC

Leytem, A.B. and Kwanyuen, P. and Plumstead, P.W. and Maguire, R.O. and Brake, J. (2008) Evaluation of phosphorus characterization in broiler ileal digesta, manure, and litter samples: 31P-NMR vs. HPLC. Journal of Environmental Quality. 37:494-500. 1 March 2008. ASA, CSSA, SSSA, Madison, WI.

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Abstract

Using 31-phosphorus nuclear magnetic resonance spectroscopy ((31)P-NMR) to characterize phosphorus (P) in animal manures and litter has become a popular technique in the area of nutrient management. To date, there has been no published work evaluating P quantification in manure/litter samples with (31)P-NMR compared to other accepted methods such as high performance liquid chromatography (HPLC). To evaluate the use of (31)P-NMR to quantify myo-inositol hexakisphosphate (phytate) in ileal digesta, manure, and litter from broilers, we compared results obtained from both (31)P-NMR and a more traditional HPLC method. The quantification of phytate in all samples was very consistent between the two methods, with linear regressions having slopes ranging from 0.94 to 1.07 and r(2) values of 0.84 to 0.98. We compared the concentration of total monoester P determined with (31)P-NMR with the total inositol P content determined with HPLC and found a strong linear relationship between the two measurements having slopes ranging from 0.91 to 1.08 and r(2) values of 0.73 to 0.95. This suggests that (31)P-NMR is a very reliable method for quantifying P compounds in manure/litter samples.

Item Type:	Article
NWISRL Publication Number:	1246
Subjects:	Manure > Chemistry
Depositing User:	Users 5 not found.
Date Deposited:	01 Apr 2008 14:54
Last Modified:	19 Oct 2016 19:15
Item ID:	1268
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/1268