Survey of selected antibiotic resistance genes in agricultural and non-agricultural soils in south-central Idaho

Dungan, R.S. and Strausbaugh, C.A. and Leytem, A.B. (2019) Survey of selected antibiotic resistance genes in agricultural and non-agricultural soils in south-central Idaho. FEMS Microbiology Ecology. 24 May 2019.

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Abstract

Agroecosystems are regions of intense agriculture production, which could be a potential hotspot for antibiotic resistance. In this study, agricultural soils (cropland, inactive cropland, pastureland, rangeland) and non-agricultural soils (recreational, residential, industrial, natural) were collected in south-central Idaho, then analyzed using quantitative real-time PCR (qPCR) to determine the occurrence and abundance of a class 1 integron-integrase gene (intI1) and six antibiotic resistance genes (ARGs): blaCTX-M-1, erm(B), sul1, tet(B), tet(M), and tet(X). All of the ARGs (except blaCTX-M-1) and intI1 were detected in some of the soils (15 to 60 detections out of 98 samples), with sul1 and intI1 being detected the most frequently. Except for a few instances, erm(B), tet(B), and tet(X) were primarily detected in the cropland soils. In addition, intI1, sul1, and tet(M) were detected more frequently in the cropland soils and in greater relative abundances on average than in all other soils. The results from this study provide evidence that intensively managed cropland soils have a resistome that is greatly altered from that of other agricultural and native soils.

Item Type: Article
NWISRL Publication Number: 1651
Subjects: Soil > Chemistry
Soil > Soil quality
Soil
Depositing User: Michelle Wayment
Date Deposited: 28 May 2019 22:13
Last Modified: 28 May 2019 22:13
Item ID: 1695
URI: https://eprints.nwisrl.ars.usda.gov/id/eprint/1695

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