Phosphorus compounds in sequential extracts of animal manures: Chemical speciation and a novel fractionation procedure

Compuestos de Fósforo en Extractos Secuenciales de Abono Animal: Evolución de Especies Química y un Procedimiento Novel Fraccional

Turner, Benjamin L. and Leytem, April B. (2004) Phosphorus compounds in sequential extracts of animal manures: Chemical speciation and a novel fractionation procedure. Environmental Science & Technology. 38:6101-6108.

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Abstract

Pollution of water bodies by phosphorus in runoff from
soil amended with animal manures is one of the greatest
threats to water quality in developed countries. The
environmental fate of manure phosphorus is determined
in part by its chemical composition, yet extraction procedures
to assess this are poorly developed and provide no
structural information. We used solution 31 P NMR
spectroscopy to quantify phosphorus compounds in
sequential extracts of three contrasting manures (broiler
litter, beef-cattle manure, swine manure). Using a procedure
originally developed for soils, but commonly applied to
manures, phosphorus was extracted sequentially with
deionized water, 0.5 M NaHCO 3, 0.1 M NaOH, and 0.5 M
HCI. Water and NaHCO 3 extracted readily soluble compounds,
including phosphate, phospholipids, DNA, and simple
phosphate monoesters, which are mobile in soil and
biologically available. In contrast, NaOH and HCI extracted
poorly soluble compounds, including phytic acid (myoinositol
hexakisphosphate). The latter is immobile in soil
and of limited biological availability. Based on these results,
we developed a simplified two-step fractionation procedure
involving extraction of readily soluble phosphorus in 0.5
M NaHCO3 followed by extraction of stable phosphorus in
a solution containing 0.5 M NaOH and 50 mM EDTA.
This revised procedure separates manure phosphorus
into structurally defined fractions with environmental
relevance and will facilitate research on this important
aspect of environmental science.

Item Type:	Article
NWISRL Publication Number:	1142
Subjects:	Manure > Chemistry Mass Import - autoclassified (may be erroneous)
Depositing User:	Users 5 not found.
Date Deposited:	20 Nov 2010 21:49
Last Modified:	03 Nov 2016 16:07
Item ID:	5
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/5