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Identification of scyllo-inositol phosphates in soil by solution phosphorus-31 nuclear magnetic resonance spectroscopy

Identificación de Fosfatos scyllo-Inositol en el Suelo por medio de Espectroscopia de Resonancia Magnética Nuclear de la Solución Fósforo-31

Turner, Benjamin L and Richardson, Alan E. (2004) Identification of scyllo-inositol phosphates in soil by solution phosphorus-31 nuclear magnetic resonance spectroscopy. Soil Science Society of America Journal. 68:802-808.

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A large proportion of the organic P in soils can occur as scyllo-inositol
phosphates. These compounds are rarely detected elsewhere
in nature and remain poorly understood, partly because conventional
procedures for their determination are lengthy and erroneous. We
report a straightforward procedure for the determination of scyllo-inositol
phosphates in soil extracts using solution 31P nuclear magnetic
resonance (NMR) spectroscopy. Solution 31P NMR chemical shifts of
a range of synthetic scyllo-inositol phosphate esters were determined
in alkaline solution. Of these, only the signal corresponding to scyllo-inositol
hexakisphosphate at approximately 4.2 ppm was identified
in soil NaOH–EDTA extracts, constituting between 6.5 and 9.8% of
the NaOH–EDTA extracted P. This signal has been previously assigned
to choline phosphate, but we confirmed it to be an inositol
phosphate using hypobromite oxidation, a procedure that destroys
all organic matter except inositol phosphates. Lower order scyllo-inositol
phosphate esters were not identified in the extracts studied
here, and literature reports suggest that they probably occur in insufficient
concentrations to be detected by this procedure. The identification
of scyllo-inositol hexakisphosphate in soils and other environmental
samples will allow its quantification in a range of environments,
and facilitate research into the origins and function of this enigmatic

Item Type: Article
NWISRL Publication Number: 1125
Subjects: Soil > Chemistry > Phosphorous
Research methodology
Mass Import - autoclassified (may be erroneous)
Depositing User: Dan Stieneke
Date Deposited: 20 Nov 2010 21:50
Last Modified: 07 Nov 2016 16:43
Item ID: 179