Separation of soil organic phosphorus compounds using reverse-phase ion-pair chromatography

La Separación de Compuestos de Suelo de Fósforo Orgánico Utilizando Cromatografía de Pares-Iónica de Fase-Reversible

Leytem, April B. and Mikkelsen, Robert L. (2003) Separation of soil organic phosphorus compounds using reverse-phase ion-pair chromatography. Communications in Soil Science and Plant Analysis. 34(9&10):1393-1406.

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Abstract

Methods were developed for the extraction and separation of soil organic
phosphorus compounds using reverse-phase ion-pair chromatography
(RP-ICP). Nucleotides (ATP, ADP, and AMP) were separated using a
mobile phase of 15 mM TBAHS, 15 mM KH2PO4, and 7% acetonitrile.
Inositol hexakisphosphate was separated using a mobile phase
composition of 0.05 M formic acid:methanol (49:51 v/v) and
1.5 mL/100 mL of TBAOH. Extraction procedures were developed for
the nucleotides which would be compatible with the RP-ICP system
developed for their separation.

Item Type:	Article
NWISRL Publication Number:	1100
Subjects:	Soil > Chemistry > Phosphorous Research methodology Mass Import - autoclassified (may be erroneous)
Depositing User:	Users 5 not found.
Date Deposited:	20 Nov 2010 21:50
Last Modified:	09 Nov 2016 16:16
Item ID:	159
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/159