Beet curly top resistance in USDA-ARS Plant Introductions, 2012

Panella, Leonard W. and Strausbaugh, C.A. (2013) Beet curly top resistance in USDA-ARS Plant Introductions, 2012. Plant Disease Management Reports. 7:FC121.

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Abstract

Thirty sea beet (Beta vulgaris subsp. maritima (L.) Arcang) and beet (Beta vulgaris subsp. vulgaris L.) plant introduction (PI) accessions from the Beta collection of the USDA-ARS National Plant Germplasm System were screened for resistance to Beet severe curly top virus (BSCTV) and other closely related Curtovirus species in 2012. Commercial cultivars Monohikari and HM PM90 and Betaseed, Inc. germplasm line G6040 were included as susceptible and resistant checks. The curly top evaluation was conducted at the USDA-ARS North Farm in Kimberly, ID which has Portneuf silt loam soil and had been in alfalfa in 2011. The field was plowed in the fall and in the spring, fertilized (90 lb N and 110 lb P2O5/A) on 16 Apr 12, sprayed with Ethotron (2 pt/A), and roller harrowed. The germplasm was planted (density of 142,560 seeds/A) on 21 May. The plots were two rows 10 ft long with 22-in row spacing and arranged in a randomized complete block design with three replications. The fields were sprinkler irrigated and hand weeded as necessary. Plant populations were thinned to about 47,500 plants/A on 19 Jun. Plants were inoculated at the four to six leaf growth stage on 22 Jun with six viruliferous beet leafhoppers per plant. The beet leafhoppers were moved twice a day (right after sunrise and just before sunset) for one week by dragging a tarp through the field. The plants were sprayed with Lorsban 4E (1.5 pints/A) on 4 Jul to kill the beet leafhoppers. The plots were rated for foliar symptom development on 10 Jul using a scale of 0-9 (0 = healthy and 9 = dead; Mumford 1974), with disease index (DI) treated as a continuous variable. Data were analyzed using the general linear models procedure (Proc GLM-SAS), and Fisher’s protected least significant difference was used for mean comparisons. Disease development was uniform and other disease problems were not evident in the plot area. The disease pressure in the test was severe with good disease development in the more susceptible lines. Most of the lines were not significantly different from the most resistant check. These accessions will be retested and, if the resistance is confirmed, entered into USDA-ARS breeding programs to enhance sugar beet germplasm with increased resistance to Beet severe curly top virus (BSCTV) and other closely related Curtovirus species. These data will be entered into the USDA-ARS, NPGS GRIN database (http://www.ars-grin.gov/npgs/index.html).

Item Type:	Article
NWISRL Publication Number:	1488
Subjects:	Irrigated crops > Sugarbeet > Curly top Irrigated crops > Sugarbeet
Depositing User:	Users 6 not found.
Date Deposited:	04 Mar 2014 00:18
Last Modified:	04 Mar 2014 00:18
Item ID:	1529
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/1529