The characterization and composition of bacterial communities in soils blended with spent foundry sand

Dungan, R.S. and Kim, J. and Weon, H. and Leytem, A.B. (2009) The characterization and composition of bacterial communities in soils blended with spent foundry sand. Annals of Microbiology. 59(2):239-246.

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Abstract

The purpose of this research was to characterize the structure and composition of bacterial communities in
sandy loam and silty clay soils amended with 30% spent sand from iron, aluminum, and steel foundries. All spent foundry
sand (SFS) blends were grown with or without perennial ryegrass and samples were collected at 4 weeks and 6 months.
Regions of the 16S rRNA gene were amplified using PCR and subsequently analyzed by DGGE and sequenced for bacterial
identification and phylogenetic classification. Cluster analyses of PCR-DGGE banding patterns revealed that SFS blends
from week 4 and month 6 produced unique clusters, with most ryegrass treatments clustering away from those without
ryegrass. The diversity of the bacterial community revealed that it was generally higher in the SFS blends without ryegrass.
By month 6 in treatments without ryegrass, the diversity in the sandy loam blends was similar to the control, while the
diversity in all silty clay blends was greater than the control. Phylogenetic analysis of bacterial isolates (total of 309) from
the SFS blends showed that they were dominated by Actinobacteria (46%), Proteobacteria (29%), and Bacilli (20%), with
fewer numbers belonging to Bacteroidetes (5%). While the addition of SFS to soil does bring about bacterial community
level changes, these changes are similar to that of blending soil with clean silica sand.

Item Type:	Article
NWISRL Publication Number:	1316
Subjects:	Soil Soil > Soil quality
Depositing User:	Users 6 not found.
Date Deposited:	30 Nov 2009 22:03
Last Modified:	17 Oct 2016 15:49
Item ID:	1339
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/1339