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Curly top survey in the Western United States

Strausbaugh, C.A. and Wintermantel, W.M. and Gillen, A.M. and Eujayl, Imad A. (2008) Curly top survey in the Western United States. Phytopathology. 99(11):1212-1217. November 2008.

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Curly top in sugar beet continues to be a challenging disease to control
in the western United States. To aid in development of host resistance and
management options, the curtovirus species composition was investigated
by sampling 246 commercial fields along with nursery and field trials in
the western United States. DNA was isolated from leaf samples and the
species were identified using species-specific polymerase chain reaction
primers for the C1 gene. Amplicons from 79 isolates were also sequenced
to confirm identifications. Beet severe curly top virus (BSCTV) and Beet
mild curly top virus (BMCTV) were widely distributed throughout the
western United States, while only a few isolates of Beet curly top virus
(BCTV) were found. In phylogenetic analysis, BSCTV, BMCTV, and
BCTV isolates formed distinct groups in the dendrogram. Seven isolates
not amplifiable with species-specific primers did amplify with curly top
coat protein primers, indicating novel curtovirus species or strains may be
present. Given the wide host range of the viruses responsible for curly
top, frequent co-infections, and genetic diversity within and among
species, establishing better host resistance, and controlling curly top will
continue to be a challenge.

Item Type: Article
NWISRL Publication Number: 1273
Subjects: Irrigated crops > Sugarbeet > Curly top
Depositing User: Users 6 not found.
Date Deposited: 16 Dec 2008 19:13
Last Modified: 18 Oct 2016 15:35
Item ID: 1296