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Transgenic sugar beet cultivars evaluated for resistance to bacterial root rot in Idaho, 2007

Strausbaugh, C.A. and Eujayl, Imad A. and Foote, P. (2008) Transgenic sugar beet cultivars evaluated for resistance to bacterial root rot in Idaho, 2007. Plant Disease Management Reports. 2:FC108. 21 July 2008.

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Thirty-three transgenic (glyphosate resistant) sugar beet cultivars were grown in a commercial sprinkler-irrigated
sugar beet field near American Falls, ID where potatoes were grown in 2006. The plots were planted on 30 Apr to a density
of 352,272 seeds/ha, and thinned to 88,068 plants/ha on 12 Jun. Plots were four rows (0.56-m row spacing) and 10.5 m long.
The experimental design was a randomized complete block design with eight replications. The crop was managed according
to standard cultural practices. The field trial was free of foliar and root disease symptoms. Four roots from one plot for each
cultivar from the same replication were hand topped and harvested on 1 Oct. The roots were then placed in a cold room at
3°C and 90% relative humidity until they were assayed on 3 Feb 08. The roots were washed, dipped in 0.6% sodium
hypochlorite solution for 1 min, rinsed in sterile reverse osmosis water, and then air dried in a laminar hood. A cross section
from the middle of the root 8-10 mm thick and 45 to 70 mm in diameter was cut from each root and placed in a Petri dish on
sterile filter paper moistened with sterile well water. A 2 mm diameter and 3 mm deep hole was created with a sterile tooth
pick in the center of the root slice. A sterile tooth pick was then dipped in a 48 hr old culture of Leuconostoc mesenteroides
subsp. dextranicum B322 grown on MRS media at 30°C and placed in the hole along with a drop of sterile well water. Four
additional root slices from HM090026 served as the uninoculated check (no bacteria inoculated). The root slice/Petri dish
combination was placed in a plastic bag and incubated at 30°C. The experiment was a randomized complete block design
with 4 replications (1 root slice = 1 replication for each cultivar). The diameter of rotted root area was recorded after 72 and
96 hr. Bacteria from the 10 largest lesions in each replication were streaked onto MRS to prove only L. mesenteroides was
present. Data were analyzed using the general linear models procedure (Proc GLM-SAS), and Fisher’s protected least
significant difference was used for mean comparisons.

Item Type: Article
NWISRL Publication Number: 1264
Subjects: Irrigated crops > Sugarbeet
Depositing User: Users 6 not found.
Date Deposited: 13 Aug 2008 20:28
Last Modified: 19 Oct 2016 18:50
Item ID: 1287