NWISRL PublicationsThe Effect of Leaf Water Variables on Ice Nucleating Pseudomonas syringae in BeansJ.W.CaryauthorS.E.LindowauthorPinto bean seedlings 'UI 114' (Phaseolus vulgaris L.) were subjected to temperatures between – 2° and – 5°C for periods ranging from 0.5 to 12 hr. The plants that were not sprayed with a suspension of the nucleating Pseudomonas syringae bacteria and those that were water-stressed to near wilting were most resistant to ice nucleation. Plants with dry leaf surfaces were much more apt to supercool than those with distilled water droplets on their leaves, whether inoculated with the bacteria or not. Spraying the freeze-dried bacteria suspended in distilled water on the leaves increased wettability and dew formation on the leaf surfaces. Tests with an oxytetracycline preparation, which also increased wetting, suggested that a hydrophobic leaf surface helps delay ice formation. Use of wetting agents in leaf sprays may be counterproductive so far as supercooling stability is concerned. It is obvious that leaf water relations interact with bacterial ice nucleation.Bean / dry beanMass Import - autoclassified (may be erroneous)1986Article

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