NWISRL PublicationsA novel technique for the pre-concentration and extraction of inositol hexakisphosphate from soil extracts with determination by phosphorus-31 nuclear magnetic resonanceBenjamin L.TurnerauthorIan D.McKelvieauthorInositol hexakisphosphate (IP6 ) is often the dominant form of soil organic phosphorus (P), but is rarely investigated because of the analytical difficulties encountered in its extraction, separation, and detection in environmental samples. In particular, recent advances in the study of soil organic P with 31 P nuclear magnetic resonance (NMR) have been of limited use for the study of IP6 , because the technique does not discriminate between IP6 and other forms of P. This was addressed by developing a novel analytical procedure using the retentive properties of gel-filtration gels for IP6, which allows the combined selective extraction and pre-concentration of IP 6 from soil extracts with determination by 31 P NMR. While the technique is still in the developmental stage, the results demonstrate that the gel does not interfere with 31 13 NMR analysis and retains IP6 to concentrations well above those required to give clear spectral signals. The technique has considerable potential for application to the study of IP6 in soil extracts and water samples and, with development, could help to answer fundamental questions regarding the dynamics of organic P in the environment.PhosphorousResearch methodologyMass Import - autoclassified (may be erroneous)2002Article

For work being deposited by its own author: In self-archiving this collection of files and associated bibliographic metadata, I grant NWISRL Publications the right to store them and to make them permanently available publicly for free on-line. I declare that this material is my own intellectual property and I understand that NWISRL Publications does not assume any responsibility if there is any breach of copyright in distributing these files or metadata. (All authors are urged to prominently assert their copyright on the title page of their work.)

For work being deposited by someone other than its author: I hereby declare that the collection of files and associated bibliographic metadata that I am archiving at NWISRL Publications) is in the public domain. If this is not the case, I accept full responsibility for any breach of copyright that distributing these files or metadata may entail.

Clicking on the deposit button indicates your agreement to these terms.