Beet curly top resistance of USDA-ARS National Plant Germplasm System Plant Introductions, 2009.

Panella, Leonard W. and Strausbaugh, C.A. (2011) Beet curly top resistance of USDA-ARS National Plant Germplasm System Plant Introductions, 2009. Plant Disease Management Reports. 5:p. FC065.

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Abstract

Thirty wild beet (Beta vulgaris subsp. maritima (L.) Arcang) accessions from the Beta Collection of the USDA-ARS National Plant Germplasm System were screened for resistance to Beet severe curly top virus (BSCTV) in 2009. The curly top evaluation was conducted at the USDA-ARS North Farm in Kimberly, ID which had been in beans in 2008. The field was plowed in the fall, fertilized (75 lb N/A and 75 lb P2O5/A) on 22 Apr 09, sprayed with Ethotron (2 pt/A), and roller harrowed. The germplasm was planted (density of about 143,000 seeds/A) on 18 May. The plots were two rows 10 ft long with 22-in row spacing and arranged in a randomized complete block design with three replications. A resistant breeding line from Betaseed, Inc., G6040, was included as a resistant check. The fields were sprinkler irrigated and hand weeded as necessary. Plant populations were thinned to about 47,500 plants/A on 19 Jun. Plants were inoculated at the four to six leaf growth stage on 23 Jun with six viruliferous beet leafhoppers per plant. The beet leafhoppers were moved twice a day (right after sunrise and just before sunset) for one week by dragging a tarp through the field. The plants were sprayed with Lorsban 4E (1.5 pints/A) on 7 Jul to kill the beet leafhoppers. The plots were rated for foliar symptom development using a scale of 0-9 where 0 = healthy and 9 = dead (Mumford, D.L. 1974. Procedure for inducing curly top epidemics in field plots. J. Am. Soc. Sugar Beet Technol. 18:20-23), with disease index (DI) treated as a continuous variable. Data were analyzed using the general linear models procedure (Proc GLM-SAS), and least significant difference was used for mean comparisons.
Disease development was uniform and severe. Other disease problems were not evident in the plot area. The PIs were a combination of annual and biennial plant types. The resistant check was significantly more resistant than any of the tested germplasms. None of the lines tested appeared to be resistant to BSCTV. The two best germplasm were Beta vulgaris subspecies maritima lines, both of which contained biennial plants. However their scores were much higher than the resistant check and do not seem to contain resistance to BSCTV.

Item Type:	Article
NWISRL Publication Number:	1389
Subjects:	Irrigated crops > Sugarbeet > Curly top Irrigated crops > Sugarbeet > Genetics Irrigated crops > Sugarbeet
Depositing User:	Users 6 not found.
Date Deposited:	03 Aug 2011 20:06
Last Modified:	04 Mar 2014 15:20
Item ID:	1419
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/1419