Qualitative and quantitative methodologies for determination of airborne microorganisms at concentrated animal-feeding operations

Dungan, R.S. and Leytem, A.B. (2009) Qualitative and quantitative methodologies for determination of airborne microorganisms at concentrated animal-feeding operations. World Journal of Microbiology and Biotechnology. 25:1505-1518. (In Press)

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Abstract

The generation of airborne microorganisms
from concentrated animal-feeding operations (CAFOs) is a
concern from a human and animal health perspective. To
better understand the airborne microorganisms found in
these environments, a number of collection and analytical
techniques have been utilized and will be discussed in this
review. The most commonly used bioaerosol collection
method is the liquid impingement format, which is suitable
with a number of culture-based and non-culture molecularbased
approaches, such as polymerase chain reaction.
However, the vast majority of airborne microorganism
studies conducted at CAFOs utilize culture-based analyses.
Because of the limitations often associated with culturebased
analyses, we focused our discussion on the application
of molecular-based techniques to identify and/or
quantify microorganisms, as they have promising application
in bioaerosol research. The ability to rapidly characterize
airborne microorganisms will help to ensure
protection of public and environmental health.

Item Type:	Article
NWISRL Publication Number:	1314
Subjects:	Manure Animal
Depositing User:	Users 6 not found.
Date Deposited:	30 Nov 2009 21:57
Last Modified:	17 Oct 2016 15:51
Item ID:	1337
URI:	https://eprints.nwisrl.ars.usda.gov/id/eprint/1337